| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Development of a qPCR Method for the Identification and Quantification of Two Closely Related Tuna Species, Bigeye Tuna ( Thunnus obesus ) and Yellowfin Tuna ( Thunnus albacares ), in Canned Tuna
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| Autores: |
Bojolly, Daline
Doyen, Perine
Le Fur, Bruno
Christaki, Urania
Verrez-bagnis, Veronique
Grard, Thierry
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| Data: |
2017-02
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| Ano: |
2017
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| Palavras-chave: |
Tuna
Authentication
Quantification
Canned products
TaqMan
QPCR
Species identification
Bigeye tuna (Thunnus obesus)
Yellowfin tuna (Thunnus albacares)
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| Resumo: |
Bigeye tuna (Thunnus obesus) and yellowfin tuna (Thunnus albacares) are among the most widely used tuna species for canning purposes. Not only substitution but also mixing of tuna species is prohibited by the European regulation for canned tuna products. However, as juveniles of bigeye and yellowfin tunas are very difficult to distinguish, unintentional substitutions may occur during the canning process. In this study, two mitochondrial markers from NADH dehydrogenase subunit 2 and cytochrome c oxidase subunit II genes were used to identify bigeye tuna and yellowfin tuna, respectively, utilizing TaqMan qPCR methodology. Two different qPCR-based methods were developed to quantify the percentage of flesh of each species used for can processing. The first one was based on absolute quantification using standard curves realized with these two markers; the second one was founded on relative quantification with the universal 12S rRNA gene as the endogenous gene. On the basis of our results, we conclude that our methodology could be applied to authenticate these two closely related tuna species when used in a binary mix in tuna cans.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00371/48217/48403.pdf
DOI:10.1021/acs.jafc.6b04713
https://archimer.ifremer.fr/doc/00371/48217/
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| Editor: |
Amer Chemical Soc
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| Formato: |
application/pdf
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| Fonte: |
Journal Of Agricultural And Food Chemistry (0021-8561) (Amer Chemical Soc), 2017-02 , Vol. 65 , N. 4 , P. 913-920
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| Direitos: |
2017 American Chemical Society
info:eu-repo/semantics/openAccess
restricted use
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